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DOI:
:2018,31(12):-
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基于分子间裂分G-四链体-氯化血红素DNA酶自组装纳米线的“Turn-on”型汞离子传感研究
张何, 赵智粮, 傅昕, 王青
(湖南工程学院化学化工学院)
"Turn-on" Sensor for Detection of Mercury Ion through Autonomous Assembly of Intermolecular Splitting G-Quardruplex-Hemin DNAzyme Nanowires
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中文摘要: 以聚苯乙烯微球为载体,利用杂交链式反应,发展了一种基于T-Hg2+-T特异性识别及串联分子间裂分G-四链体-血红素DNA酶纳米线催化信号放大的Hg2+“Turn on”型高灵敏生物传感器。当汞离子存在时,“T-Hg2+-T”特殊结构的形成促使微球表面修饰的富T探针打开并捕获设计的发夹探针P1,由此引发与发夹探针P2之间的杂交链式反应。由于P1、P2的5’游离末端及3’游离末端都包含富G序列,通过杂交链式反应相互拉近并串联排列,在微球表面折叠形成包含串联分子间裂分G-四链体-血红素DNA酶的自组装DNA纳米线。在最优实验条件下,传感器对Hg2+的线性检测范围为2 pmol/L-1 nmol/L,检出限为1.5 pmol/L;当试样中的共存离子大量存在时,传感器对Hg2+仍然具有高的选择性。此方法检测Hg2+具有良好的选择性、灵敏度及稳定性。
Abstract:Based on T-Hg2+-T structure for specific recognition and the tandem intermolecular splitting G-quadruplex-hemin DNAzymes nanowires for catalytic signal amplification, a highly sensitive "Turn-on" biosensor for detection of Hg2+ was developed on the polystyrene microbeads using the hybridization chain reaction. When Hg2+ was present in reaction system, the formation of T-Hg2+-T special structure prompted T-rich DNA probes modified on the microbeads to open and capture the designed hairpin probe P1, which triggered the hybridization chain reaction between hairpin probe P1 and hairpin probe P2. Since the 5’ free terminal and the 3’ free terminal of hairpin probe P1 and P2 both contain the G-rich sequences which are contacted closely with each other and arranged in series through hybridization chain reaction, and the neighbouring G-rich sequences further fold to form autonomously assembled nanowires containing tandem intermolecular splitting G-quardruplex-hemin DNAzyme on the interface of polystyrene microspheres. Under the optimal experimental conditions, the linear range for detection of Hg2+ was 2 pmol/L-1 nmol/L, and the detection limit was 1.5 pmol / L. The sensor still has a high selectivity to Hg2+ when the coexisting ions in the sample are abundant. The sensor had good selectivity, sensitivity, and stability for Hg2+ analysis.
文章编号:cg18000475     中图分类号:    文献标志码:
基金项目:集成化多功能可控细胞操纵及分析微流控芯片的研制
张何  赵智粮  傅昕  王青 湖南工程学院化学化工学院
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